removal rate in one round of separation. This method enables a

low-cost and rapid fabrication process for the bioprocessing filtra-

tion devices and is capable of separating microcarriers in size range

of 100–300 μm.

2

Materials

2.1

3D Printing

1. High resolution Digital Light Processing (DLP)/Stereolitho-

graphy

(SLR)

3D

printer

(see

Note

1),

filled

with

corresponding light-sensitive resin.

2. Scalpel for removing printed chip from the platform.

3. 100% isopropyl alcohol (IPA) (see Note 2).

4. UV curing machine.

Fig. 1 The inertial focusing of microparticles inside (a) straight microfluidic channels with square cross

sections and (b) spiral microchannels with trapezoidal cross sections. The particle equilibrium positions are

highly affected by the geometry of the channels. (c) The two methods of making the microfluidic channels for

MSCs, microcarriers separation

Bioreactor-Based Adherent Cells Harvesting from Microcarriers with 3D. . .

259